A lamin-like protein gene is down-regulated in human gastric cancer / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To clone human gastric cancer related gene and to analyze its expression profile in gastric mucosal tissues.</p><p><b>METHODS</b>Paired tumor, paratumor and non-tumor specimens from 7 gastric adenocarcinoma patients (male 4, female 3, with average age 51 +/- 18 years) were studied by means of fluorescent differential display reverse transcription polymerase chain reaction (DDRT-PCR). The differentially expressed cDNA bands of interest were cloned and analyzed by Northern blot and in situ hybridization. Thirty cases (male 23 female 7 with average age 59 +/- 8 years) of paired paraffin-embedded gastric tumor and non-tumor tissues were used in in situ hybridization analysis.</p><p><b>RESULTS</b>A gene expressed much lower in 6 out of 7 tested tumor samples than in their normal and paratumor counterparts was identified. It was named GCRG123. Northern blot analysis confirmed the differential expression. Human multiple tissue Northern blot analysis showed that GCRG123 expressed in various adult human tissues including thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Sequence analysis revealed that GCRG123 (GenBank accession number AF454554) was a lamin like protein gene. It had one open reading frame which consisted of 49 amino acids (GenBank accession number AAL61668.1). In situ hybridization analysis showed a high GCRG123 expression level in normal gastric epithelium and pylori glands, but low expression level in tumor as well as dysplasia and most intestinal metaplasia at the paratumor regions.</p><p><b>CONCLUSION</b>A lamin-like protein gene was identified in human gastric mucosa, it is down-regulated in gastric cancer and its precancerous leisions.</p>

Co-expression of immunoglobulin light chain kappa and lambda in gastric carcinoma cell / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To study the expression of immunoglobulin light chain kappa and lambda (Igkappa and Iglambda) in gastric carcinoma cell and their co-expression.</p><p><b>METHODS</b>Igkappa and Iglambda of 22 human gastric carcinoma specimens embedded in paraffin were monitored through immunohistochemical method-LSAB method.</p><p><b>RESULTS</b>Among 22 gastric carcinoma specimens, both Igkappa and Iglambda were positive in 17 (77.3%), only Igkappa was positive in 2 (9.1%), only Iglambda was positive in 1 (4.5%), both Igkappa and Iglambda negative in 2 (9.1%). The expression of Igkappa and Iglambda in human gastric carcinoma cell showed significant close correlation (chi(2) = 5.49, P < 0.05).</p><p><b>CONCLUSION</b>Co-expression of immunoglobulin light chain kappa and lambda in gastric carcinoma cell is common, which suggests that the activation mechanism of immunoglobulin gene in gastric carcinoma cell may be different from that in B-lymphocytes. Study on co-expression of immunoglobulin light chain kappa and lambda in gastric carcinoma is promising.</p>

STUDY ON THE EXPRESSION OF ICAM-1 AND TNF-? IN GASTRIC MUCOSAL CELL DURING DRUG INDUCED GASTRIC MUCOSAL INJURY IN RAT / 解放军医学杂志

To investigate the expression of ICAM 1 and TNF ? in gastric mucosal cell during drug induced mucosal injury, the authors carried out the study in SD rats with aspirin by mouth with immunohistochemical method . The results showed that ICAM 1 began to express at 30min after administration of aspirin, peaked at 1h, maintained until 6h, decreased at 12h,and expressed slightly at 24h . TNF ? began to express at 15min, peaked at 3h to 6h,decreased at 12h, and expressed significantly at 24h. There was a slight ICAM 1 and TNF ? expression in gastric mucosal cells in the control group. It was suggested that the expression of ICAM 1 and TNF ? plays an important role in the mechanisms of gastric mucosal injury .